Journal: Frontiers in Oncology

Article Title: Monocyte Chemoattractant Protein 1 Promotes VEGF-A Expression in OSCC by Activating ILK and MEK1/2 Signaling and Downregulating miR-29c

doi: 10.3389/fonc.2020.592415

Figure Lengend Snippet: Clinical significance of MCP-1 in angiogenesis of oral squamous cell carcinoma. (A–C) OSCC tissue and adjacent normal tissue data from the Oncomine database ( https://www.oncomine.org ) were analyzed for MCP-1, VEGF-A, and ANGPT2 expression. (D) IHC photographs of tissue array sections (OR601) immunostained with anti-MCP-1 or anti-VEGF-A antibody. (E, F) IHC results were scored on a scale of 1–5 for staining intensity. Levels of MCP-1 and VEGF-A expression correlated with OSCC clinical grade. **p < 0.005 , ***p < 0.0005 compared with controls. (A–C, E) are box-and-whisker plots that plot outliers as individual points (dots).

Article Snippet: The human OSCC cell line used in this study was SCC4, and was purchased from the Bioresource Collection and Research Center (BCRC) (Hsinchu, Taiwan).

Techniques: Expressing, Staining, Whisker Assay

Journal: Frontiers in Oncology

Article Title: Monocyte Chemoattractant Protein 1 Promotes VEGF-A Expression in OSCC by Activating ILK and MEK1/2 Signaling and Downregulating miR-29c

doi: 10.3389/fonc.2020.592415

Figure Lengend Snippet: MCP-1 promotes human OSCC cell angiogenesis through the VEGF-A-dependent pathway. Cells were incubated with MCP-1 (0–50 ng/ml) for 24 h and VEGF-A expression was determined by Western blot (A) , RT-qPCR (B) , and ELISA assays (C) . The CM was collected and used to treat the EPCs for 24 h. We used the tube formation assay to examine capillary-like structure formation (D) and the Transwell assay to examine cell migration (E) . * p < 0.05 compared with controls; # p < 0.05 compared with MCP-1–treated controls. Each experimental procedure was independently repeated three times, with similar results.

Article Snippet: The human OSCC cell line used in this study was SCC4, and was purchased from the Bioresource Collection and Research Center (BCRC) (Hsinchu, Taiwan).

Techniques: Incubation, Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Transwell Assay, Migration

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 is downregulated in oral squamous cell carcinoma. A Venn diagram illustrating lncRNAs derived from TCGA data and GeneCards. B Expression levels of lncRNAs in human oral keratinocytes (NHOK) and OSCC cell lines were assessed using qRT-PCR. C SLC8A1-AS1 expression in HNSC based on TCGA data. D SLC8A1-AS1 expression in OSCC based on TCGA data. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: Overexpression of SLC8A1-AS1 reduces proliferation and migration of OSCC cells. A Expression levels of SLC8A1-AS1 in OSCC cells after SLC8A1-AS1 overexpression was assessed using qRT-PCR. B Proliferation of OSCC cells was assessed using the CCK-8 assay. C Proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D Invasion of OSCC cells were measured using the Transwell assay (Scale bar = 100 μm). E Migration of OSCC cells was analyzed through a wound healing assay. F Gene and protein levels of Ki67, MCM3, E-cad, and N-cad in OSCC cells were assessed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Over Expression, Migration, Expressing, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Transwell Assay, Wound Healing Assay, Western Blot

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 functions as a sponge for hsa-miR-1305. A Venn diagram illustrating the targets genes of SLC8A1-AS1. B Expression levels of miR-1305 in HNSC based on TCGA data. C Expression levels of miR-1305 in OSCC based on TCGA data. D Expression levels of miR-1305 in NHOK and OSCC cell lines were assessed using qRT-PCR. * P < 0.05 vs. NHOK group. E Predicted binding sites between SLC8A1-AS1 and miR-1305 are determined by lncRNASNP V3. F Expression levels of miR-1305 following transfection with miR-1305 mimics were assessed using the qRT-PCR. * P < 0.05 vs. mimics NC group. G The interaction between SLC8A1-AS1 and miR-1305 was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. H The interaction between SLC8A1-AS1 and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. I Expression levels of miR-1305 in OSCC cells following overexpression of SLC8A1-AS1. * P < 0.05 vs. pc-NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Transfection, Luciferase, Reporter Gene Assay, Pull Down Assay, Over Expression

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: ESRRG is the target protein of miR-1305. A A Venn diagram depicting the target genes of miR-1305. B The expression levels of ESRRG in HNSC based on TCGA data. C The expression levels of ESRRG in OSCC based on TCGA data. D The mRNA expression levels of ESRRG in NHOK and OSCC cell lines. * P < 0.05 vs. NHOK group. E The binding sites between miR-1305 and ESRRG predicted by TargetScan 8.0. F The target relationship between miR-1305 and ESRRG was validated using a dual-luciferase reporter gene assay. * P < 0.05 vs. mimics NC group. G The interaction between ESRRG and miR-1305 was confirmed through an RNA pull-down assay. * P < 0.05 vs. Bio-NC group. H The expression levels of ESRRG in OSCC cells after miR-1305 overexpression. * P < 0.05 vs. pc-NC group. H The expression levels of ESRRG in OSCC cells after SLC8A1-AS1 overexpression. * P < 0.05 vs. mimics NC group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Binding Assay, Luciferase, Reporter Gene Assay, Pull Down Assay, Over Expression

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 inhibits the proliferation and invasion of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The expression levels of ESRRG in OSCC cells were assessed using the qRT-PCR and western blot. B The proliferation of OSCC cells was assessed using the CCK-8 assay. C The proliferation of OSCC cells was evaluated using the EdU assay (Scale bar = 50 μm). D The invasion of OSCC cells were measured using Transwell assay (Scale bar = 100 μm). * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, EdU Assay, Transwell Assay

Journal: Discover Oncology

Article Title: SLC8A1-AS1 inhibits oral squamous cell carcinoma by regulating the miR-1305/ESRRG axis

doi: 10.1007/s12672-025-03586-w

Figure Lengend Snippet: SLC8A1-AS1 inhibits the migration of OSCC cells by regulating the expression of miR-1305 and ESRRG. A The migration of OSCC cells was assessed using the wound healing assay. B The expression levels of Ki67, MCM2, N-cad, and E-cad in OSCC cells were analyzed using the qRT-PCR and western blot. * P < 0.05 vs. pc-NC group, # P < 0.05 vs. SLC8A1-AS1 group, & P < 0.05 vs.SLC8A1-AS1 + miR-1305 mimics group

Article Snippet: The OSCC cell lines FADU (human pharyngeal squamous cells) and SCC25 (human tongue squamous cells) were obtained from Procell Life Science & Technology Co., Ltd. NHOK cells were cultured in complete culture medium specifically designed for human oral keratinocytes (Zhong Qiao Xin Zhou Biotechnology).

Techniques: Migration, Expressing, Wound Healing Assay, Quantitative RT-PCR, Western Blot